cd122 (tm-b1) – fitc Search Results


90
Miltenyi Biotec pe conjugated anti cd122 tm b1
Pe Conjugated Anti Cd122 Tm B1, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Becton Dickinson fitc-conjugated anti-il-2rb (cd122) mab (tm-b1)
Fitc Conjugated Anti Il 2rb (Cd122) Mab (Tm B1), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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99
Thermo Fisher cd122 fitc
Helios expression in CD4 + and CD8 + Tregs from MPJ and LPR mice. (A) Representative flow cytometry plots pregated on splenic CD4 + T cells (CD3 + CD8 − CD4 + ). The CD4 + Treg (CD25 + FoxP3 + ) gates are indicated. (B) Percentage of CD4 + Tregs among the CD4 + T cells. (C) Representative flow cytometry plots pregated on splenic CD8 + CD44 + T cells (CD3 + CD8 + CD44 + ), in which the CD8 + Treg <t>(CD122</t> + Ly49 + ) gates are indicated. (D) Percentage of CD8 + Treg among CD8 + CD44 + T cells. (E) Representative flow cytometry plots pregated on CD4 + Tregs with the Helios hi gate indicated. (F) Percentage of Helios hi cells among CD4 + Tregs. (G) Representative flow cytometry plots pregated on CD8 + CD127 + Tregs, with the Helios + gate indicated. (H) Percentage of Helios + cells among CD8 + Tregs. The data represent the mean ± SD: *P < 0.05, **P < 0.01; ***,P < 0.001; ****,P < 0.0001 for comparisons between MRL animals. One-way ANOVA with a Tukey multi-comparison post-test was used. Each point represents a single animal: C57BL/6, n = 3 (A, B) , 5 (C, D) , 4 (E, F) , and 5 (G, H) ; prediseased MPJ (pre.MPJ), n = 6 (A, B) , 4 (C, D) , 10 (E, F) , and 4 (G, H) ; diseased MPJ (dis.MPJ), n = 14 (A, B) , 3 (C, D) , 4 (E, F) , and 3 (G, H) ; prediseased LPR (pre.LPR), n = 10 (A, B) , 4 (C, D) , 9 (E, F) , and 4 (G, H) ; and diseased LPR (dis.LPR), n = 18 (A, B) , 6 (C, D) , 12 (E, F) , and 6 (G, H) .
Cd122 Fitc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd122+(tm-b1)+%E2%80%93+fitc/Fluorescein/pmc09244697-51-51-76
Average 99 stars, based on 1 article reviews
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96
Miltenyi Biotec nk1.1 antibody, anti-mouse
Helios expression in CD4 + and CD8 + Tregs from MPJ and LPR mice. (A) Representative flow cytometry plots pregated on splenic CD4 + T cells (CD3 + CD8 − CD4 + ). The CD4 + Treg (CD25 + FoxP3 + ) gates are indicated. (B) Percentage of CD4 + Tregs among the CD4 + T cells. (C) Representative flow cytometry plots pregated on splenic CD8 + CD44 + T cells (CD3 + CD8 + CD44 + ), in which the CD8 + Treg <t>(CD122</t> + Ly49 + ) gates are indicated. (D) Percentage of CD8 + Treg among CD8 + CD44 + T cells. (E) Representative flow cytometry plots pregated on CD4 + Tregs with the Helios hi gate indicated. (F) Percentage of Helios hi cells among CD4 + Tregs. (G) Representative flow cytometry plots pregated on CD8 + CD127 + Tregs, with the Helios + gate indicated. (H) Percentage of Helios + cells among CD8 + Tregs. The data represent the mean ± SD: *P < 0.05, **P < 0.01; ***,P < 0.001; ****,P < 0.0001 for comparisons between MRL animals. One-way ANOVA with a Tukey multi-comparison post-test was used. Each point represents a single animal: C57BL/6, n = 3 (A, B) , 5 (C, D) , 4 (E, F) , and 5 (G, H) ; prediseased MPJ (pre.MPJ), n = 6 (A, B) , 4 (C, D) , 10 (E, F) , and 4 (G, H) ; diseased MPJ (dis.MPJ), n = 14 (A, B) , 3 (C, D) , 4 (E, F) , and 3 (G, H) ; prediseased LPR (pre.LPR), n = 10 (A, B) , 4 (C, D) , 9 (E, F) , and 4 (G, H) ; and diseased LPR (dis.LPR), n = 18 (A, B) , 6 (C, D) , 12 (E, F) , and 6 (G, H) .
Nk1.1 Antibody, Anti Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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91
Biotium nk1.1 / cd161c / klrb1c, mouse(pk136)
Helios expression in CD4 + and CD8 + Tregs from MPJ and LPR mice. (A) Representative flow cytometry plots pregated on splenic CD4 + T cells (CD3 + CD8 − CD4 + ). The CD4 + Treg (CD25 + FoxP3 + ) gates are indicated. (B) Percentage of CD4 + Tregs among the CD4 + T cells. (C) Representative flow cytometry plots pregated on splenic CD8 + CD44 + T cells (CD3 + CD8 + CD44 + ), in which the CD8 + Treg <t>(CD122</t> + Ly49 + ) gates are indicated. (D) Percentage of CD8 + Treg among CD8 + CD44 + T cells. (E) Representative flow cytometry plots pregated on CD4 + Tregs with the Helios hi gate indicated. (F) Percentage of Helios hi cells among CD4 + Tregs. (G) Representative flow cytometry plots pregated on CD8 + CD127 + Tregs, with the Helios + gate indicated. (H) Percentage of Helios + cells among CD8 + Tregs. The data represent the mean ± SD: *P < 0.05, **P < 0.01; ***,P < 0.001; ****,P < 0.0001 for comparisons between MRL animals. One-way ANOVA with a Tukey multi-comparison post-test was used. Each point represents a single animal: C57BL/6, n = 3 (A, B) , 5 (C, D) , 4 (E, F) , and 5 (G, H) ; prediseased MPJ (pre.MPJ), n = 6 (A, B) , 4 (C, D) , 10 (E, F) , and 4 (G, H) ; diseased MPJ (dis.MPJ), n = 14 (A, B) , 3 (C, D) , 4 (E, F) , and 3 (G, H) ; prediseased LPR (pre.LPR), n = 10 (A, B) , 4 (C, D) , 9 (E, F) , and 4 (G, H) ; and diseased LPR (dis.LPR), n = 18 (A, B) , 6 (C, D) , 12 (E, F) , and 6 (G, H) .
Nk1.1 / Cd161c / Klrb1c, Mouse(pk136), supplied by Biotium, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
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99
Biotium cd8b, mouse(h35-17.2)
Helios expression in CD4 + and CD8 + Tregs from MPJ and LPR mice. (A) Representative flow cytometry plots pregated on splenic CD4 + T cells (CD3 + CD8 − CD4 + ). The CD4 + Treg (CD25 + FoxP3 + ) gates are indicated. (B) Percentage of CD4 + Tregs among the CD4 + T cells. (C) Representative flow cytometry plots pregated on splenic CD8 + CD44 + T cells (CD3 + CD8 + CD44 + ), in which the CD8 + Treg <t>(CD122</t> + Ly49 + ) gates are indicated. (D) Percentage of CD8 + Treg among CD8 + CD44 + T cells. (E) Representative flow cytometry plots pregated on CD4 + Tregs with the Helios hi gate indicated. (F) Percentage of Helios hi cells among CD4 + Tregs. (G) Representative flow cytometry plots pregated on CD8 + CD127 + Tregs, with the Helios + gate indicated. (H) Percentage of Helios + cells among CD8 + Tregs. The data represent the mean ± SD: *P < 0.05, **P < 0.01; ***,P < 0.001; ****,P < 0.0001 for comparisons between MRL animals. One-way ANOVA with a Tukey multi-comparison post-test was used. Each point represents a single animal: C57BL/6, n = 3 (A, B) , 5 (C, D) , 4 (E, F) , and 5 (G, H) ; prediseased MPJ (pre.MPJ), n = 6 (A, B) , 4 (C, D) , 10 (E, F) , and 4 (G, H) ; diseased MPJ (dis.MPJ), n = 14 (A, B) , 3 (C, D) , 4 (E, F) , and 3 (G, H) ; prediseased LPR (pre.LPR), n = 10 (A, B) , 4 (C, D) , 9 (E, F) , and 4 (G, H) ; and diseased LPR (dis.LPR), n = 18 (A, B) , 6 (C, D) , 12 (E, F) , and 6 (G, H) .
Cd8b, Mouse(h35 17.2), supplied by Biotium, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biotium cd3e, mouse(145-2c11)
Helios expression in CD4 + and CD8 + Tregs from MPJ and LPR mice. (A) Representative flow cytometry plots pregated on splenic CD4 + T cells (CD3 + CD8 − CD4 + ). The CD4 + Treg (CD25 + FoxP3 + ) gates are indicated. (B) Percentage of CD4 + Tregs among the CD4 + T cells. (C) Representative flow cytometry plots pregated on splenic CD8 + CD44 + T cells (CD3 + CD8 + CD44 + ), in which the CD8 + Treg <t>(CD122</t> + Ly49 + ) gates are indicated. (D) Percentage of CD8 + Treg among CD8 + CD44 + T cells. (E) Representative flow cytometry plots pregated on CD4 + Tregs with the Helios hi gate indicated. (F) Percentage of Helios hi cells among CD4 + Tregs. (G) Representative flow cytometry plots pregated on CD8 + CD127 + Tregs, with the Helios + gate indicated. (H) Percentage of Helios + cells among CD8 + Tregs. The data represent the mean ± SD: *P < 0.05, **P < 0.01; ***,P < 0.001; ****,P < 0.0001 for comparisons between MRL animals. One-way ANOVA with a Tukey multi-comparison post-test was used. Each point represents a single animal: C57BL/6, n = 3 (A, B) , 5 (C, D) , 4 (E, F) , and 5 (G, H) ; prediseased MPJ (pre.MPJ), n = 6 (A, B) , 4 (C, D) , 10 (E, F) , and 4 (G, H) ; diseased MPJ (dis.MPJ), n = 14 (A, B) , 3 (C, D) , 4 (E, F) , and 3 (G, H) ; prediseased LPR (pre.LPR), n = 10 (A, B) , 4 (C, D) , 9 (E, F) , and 4 (G, H) ; and diseased LPR (dis.LPR), n = 18 (A, B) , 6 (C, D) , 12 (E, F) , and 6 (G, H) .
Cd3e, Mouse(145 2c11), supplied by Biotium, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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NSJ Bioreagents cd8b antibody
Helios expression in CD4 + and CD8 + Tregs from MPJ and LPR mice. (A) Representative flow cytometry plots pregated on splenic CD4 + T cells (CD3 + CD8 − CD4 + ). The CD4 + Treg (CD25 + FoxP3 + ) gates are indicated. (B) Percentage of CD4 + Tregs among the CD4 + T cells. (C) Representative flow cytometry plots pregated on splenic CD8 + CD44 + T cells (CD3 + CD8 + CD44 + ), in which the CD8 + Treg <t>(CD122</t> + Ly49 + ) gates are indicated. (D) Percentage of CD8 + Treg among CD8 + CD44 + T cells. (E) Representative flow cytometry plots pregated on CD4 + Tregs with the Helios hi gate indicated. (F) Percentage of Helios hi cells among CD4 + Tregs. (G) Representative flow cytometry plots pregated on CD8 + CD127 + Tregs, with the Helios + gate indicated. (H) Percentage of Helios + cells among CD8 + Tregs. The data represent the mean ± SD: *P < 0.05, **P < 0.01; ***,P < 0.001; ****,P < 0.0001 for comparisons between MRL animals. One-way ANOVA with a Tukey multi-comparison post-test was used. Each point represents a single animal: C57BL/6, n = 3 (A, B) , 5 (C, D) , 4 (E, F) , and 5 (G, H) ; prediseased MPJ (pre.MPJ), n = 6 (A, B) , 4 (C, D) , 10 (E, F) , and 4 (G, H) ; diseased MPJ (dis.MPJ), n = 14 (A, B) , 3 (C, D) , 4 (E, F) , and 3 (G, H) ; prediseased LPR (pre.LPR), n = 10 (A, B) , 4 (C, D) , 9 (E, F) , and 4 (G, H) ; and diseased LPR (dis.LPR), n = 18 (A, B) , 6 (C, D) , 12 (E, F) , and 6 (G, H) .
Cd8b Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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NSJ Bioreagents gm130 antibody / golga2
Helios expression in CD4 + and CD8 + Tregs from MPJ and LPR mice. (A) Representative flow cytometry plots pregated on splenic CD4 + T cells (CD3 + CD8 − CD4 + ). The CD4 + Treg (CD25 + FoxP3 + ) gates are indicated. (B) Percentage of CD4 + Tregs among the CD4 + T cells. (C) Representative flow cytometry plots pregated on splenic CD8 + CD44 + T cells (CD3 + CD8 + CD44 + ), in which the CD8 + Treg <t>(CD122</t> + Ly49 + ) gates are indicated. (D) Percentage of CD8 + Treg among CD8 + CD44 + T cells. (E) Representative flow cytometry plots pregated on CD4 + Tregs with the Helios hi gate indicated. (F) Percentage of Helios hi cells among CD4 + Tregs. (G) Representative flow cytometry plots pregated on CD8 + CD127 + Tregs, with the Helios + gate indicated. (H) Percentage of Helios + cells among CD8 + Tregs. The data represent the mean ± SD: *P < 0.05, **P < 0.01; ***,P < 0.001; ****,P < 0.0001 for comparisons between MRL animals. One-way ANOVA with a Tukey multi-comparison post-test was used. Each point represents a single animal: C57BL/6, n = 3 (A, B) , 5 (C, D) , 4 (E, F) , and 5 (G, H) ; prediseased MPJ (pre.MPJ), n = 6 (A, B) , 4 (C, D) , 10 (E, F) , and 4 (G, H) ; diseased MPJ (dis.MPJ), n = 14 (A, B) , 3 (C, D) , 4 (E, F) , and 3 (G, H) ; prediseased LPR (pre.LPR), n = 10 (A, B) , 4 (C, D) , 9 (E, F) , and 4 (G, H) ; and diseased LPR (dis.LPR), n = 18 (A, B) , 6 (C, D) , 12 (E, F) , and 6 (G, H) .
Gm130 Antibody / Golga2, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation mouse il-15r alpha biotinylated antibody
Helios expression in CD4 + and CD8 + Tregs from MPJ and LPR mice. (A) Representative flow cytometry plots pregated on splenic CD4 + T cells (CD3 + CD8 − CD4 + ). The CD4 + Treg (CD25 + FoxP3 + ) gates are indicated. (B) Percentage of CD4 + Tregs among the CD4 + T cells. (C) Representative flow cytometry plots pregated on splenic CD8 + CD44 + T cells (CD3 + CD8 + CD44 + ), in which the CD8 + Treg <t>(CD122</t> + Ly49 + ) gates are indicated. (D) Percentage of CD8 + Treg among CD8 + CD44 + T cells. (E) Representative flow cytometry plots pregated on CD4 + Tregs with the Helios hi gate indicated. (F) Percentage of Helios hi cells among CD4 + Tregs. (G) Representative flow cytometry plots pregated on CD8 + CD127 + Tregs, with the Helios + gate indicated. (H) Percentage of Helios + cells among CD8 + Tregs. The data represent the mean ± SD: *P < 0.05, **P < 0.01; ***,P < 0.001; ****,P < 0.0001 for comparisons between MRL animals. One-way ANOVA with a Tukey multi-comparison post-test was used. Each point represents a single animal: C57BL/6, n = 3 (A, B) , 5 (C, D) , 4 (E, F) , and 5 (G, H) ; prediseased MPJ (pre.MPJ), n = 6 (A, B) , 4 (C, D) , 10 (E, F) , and 4 (G, H) ; diseased MPJ (dis.MPJ), n = 14 (A, B) , 3 (C, D) , 4 (E, F) , and 3 (G, H) ; prediseased LPR (pre.LPR), n = 10 (A, B) , 4 (C, D) , 9 (E, F) , and 4 (G, H) ; and diseased LPR (dis.LPR), n = 18 (A, B) , 6 (C, D) , 12 (E, F) , and 6 (G, H) .
Mouse Il 15r Alpha Biotinylated Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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NSJ Bioreagents hpv-16 antibody / e6 protein
Helios expression in CD4 + and CD8 + Tregs from MPJ and LPR mice. (A) Representative flow cytometry plots pregated on splenic CD4 + T cells (CD3 + CD8 − CD4 + ). The CD4 + Treg (CD25 + FoxP3 + ) gates are indicated. (B) Percentage of CD4 + Tregs among the CD4 + T cells. (C) Representative flow cytometry plots pregated on splenic CD8 + CD44 + T cells (CD3 + CD8 + CD44 + ), in which the CD8 + Treg <t>(CD122</t> + Ly49 + ) gates are indicated. (D) Percentage of CD8 + Treg among CD8 + CD44 + T cells. (E) Representative flow cytometry plots pregated on CD4 + Tregs with the Helios hi gate indicated. (F) Percentage of Helios hi cells among CD4 + Tregs. (G) Representative flow cytometry plots pregated on CD8 + CD127 + Tregs, with the Helios + gate indicated. (H) Percentage of Helios + cells among CD8 + Tregs. The data represent the mean ± SD: *P < 0.05, **P < 0.01; ***,P < 0.001; ****,P < 0.0001 for comparisons between MRL animals. One-way ANOVA with a Tukey multi-comparison post-test was used. Each point represents a single animal: C57BL/6, n = 3 (A, B) , 5 (C, D) , 4 (E, F) , and 5 (G, H) ; prediseased MPJ (pre.MPJ), n = 6 (A, B) , 4 (C, D) , 10 (E, F) , and 4 (G, H) ; diseased MPJ (dis.MPJ), n = 14 (A, B) , 3 (C, D) , 4 (E, F) , and 3 (G, H) ; prediseased LPR (pre.LPR), n = 10 (A, B) , 4 (C, D) , 9 (E, F) , and 4 (G, H) ; and diseased LPR (dis.LPR), n = 18 (A, B) , 6 (C, D) , 12 (E, F) , and 6 (G, H) .
Hpv 16 Antibody / E6 Protein, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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NSJ Bioreagents ox40 antibody / cd134
Helios expression in CD4 + and CD8 + Tregs from MPJ and LPR mice. (A) Representative flow cytometry plots pregated on splenic CD4 + T cells (CD3 + CD8 − CD4 + ). The CD4 + Treg (CD25 + FoxP3 + ) gates are indicated. (B) Percentage of CD4 + Tregs among the CD4 + T cells. (C) Representative flow cytometry plots pregated on splenic CD8 + CD44 + T cells (CD3 + CD8 + CD44 + ), in which the CD8 + Treg <t>(CD122</t> + Ly49 + ) gates are indicated. (D) Percentage of CD8 + Treg among CD8 + CD44 + T cells. (E) Representative flow cytometry plots pregated on CD4 + Tregs with the Helios hi gate indicated. (F) Percentage of Helios hi cells among CD4 + Tregs. (G) Representative flow cytometry plots pregated on CD8 + CD127 + Tregs, with the Helios + gate indicated. (H) Percentage of Helios + cells among CD8 + Tregs. The data represent the mean ± SD: *P < 0.05, **P < 0.01; ***,P < 0.001; ****,P < 0.0001 for comparisons between MRL animals. One-way ANOVA with a Tukey multi-comparison post-test was used. Each point represents a single animal: C57BL/6, n = 3 (A, B) , 5 (C, D) , 4 (E, F) , and 5 (G, H) ; prediseased MPJ (pre.MPJ), n = 6 (A, B) , 4 (C, D) , 10 (E, F) , and 4 (G, H) ; diseased MPJ (dis.MPJ), n = 14 (A, B) , 3 (C, D) , 4 (E, F) , and 3 (G, H) ; prediseased LPR (pre.LPR), n = 10 (A, B) , 4 (C, D) , 9 (E, F) , and 4 (G, H) ; and diseased LPR (dis.LPR), n = 18 (A, B) , 6 (C, D) , 12 (E, F) , and 6 (G, H) .
Ox40 Antibody / Cd134, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Helios expression in CD4 + and CD8 + Tregs from MPJ and LPR mice. (A) Representative flow cytometry plots pregated on splenic CD4 + T cells (CD3 + CD8 − CD4 + ). The CD4 + Treg (CD25 + FoxP3 + ) gates are indicated. (B) Percentage of CD4 + Tregs among the CD4 + T cells. (C) Representative flow cytometry plots pregated on splenic CD8 + CD44 + T cells (CD3 + CD8 + CD44 + ), in which the CD8 + Treg (CD122 + Ly49 + ) gates are indicated. (D) Percentage of CD8 + Treg among CD8 + CD44 + T cells. (E) Representative flow cytometry plots pregated on CD4 + Tregs with the Helios hi gate indicated. (F) Percentage of Helios hi cells among CD4 + Tregs. (G) Representative flow cytometry plots pregated on CD8 + CD127 + Tregs, with the Helios + gate indicated. (H) Percentage of Helios + cells among CD8 + Tregs. The data represent the mean ± SD: *P < 0.05, **P < 0.01; ***,P < 0.001; ****,P < 0.0001 for comparisons between MRL animals. One-way ANOVA with a Tukey multi-comparison post-test was used. Each point represents a single animal: C57BL/6, n = 3 (A, B) , 5 (C, D) , 4 (E, F) , and 5 (G, H) ; prediseased MPJ (pre.MPJ), n = 6 (A, B) , 4 (C, D) , 10 (E, F) , and 4 (G, H) ; diseased MPJ (dis.MPJ), n = 14 (A, B) , 3 (C, D) , 4 (E, F) , and 3 (G, H) ; prediseased LPR (pre.LPR), n = 10 (A, B) , 4 (C, D) , 9 (E, F) , and 4 (G, H) ; and diseased LPR (dis.LPR), n = 18 (A, B) , 6 (C, D) , 12 (E, F) , and 6 (G, H) .

Journal: Frontiers in Immunology

Article Title: Helios Expression Is Downregulated on CD8 + Treg in Two Mouse Models of Lupus During Disease Progression

doi: 10.3389/fimmu.2022.922958

Figure Lengend Snippet: Helios expression in CD4 + and CD8 + Tregs from MPJ and LPR mice. (A) Representative flow cytometry plots pregated on splenic CD4 + T cells (CD3 + CD8 − CD4 + ). The CD4 + Treg (CD25 + FoxP3 + ) gates are indicated. (B) Percentage of CD4 + Tregs among the CD4 + T cells. (C) Representative flow cytometry plots pregated on splenic CD8 + CD44 + T cells (CD3 + CD8 + CD44 + ), in which the CD8 + Treg (CD122 + Ly49 + ) gates are indicated. (D) Percentage of CD8 + Treg among CD8 + CD44 + T cells. (E) Representative flow cytometry plots pregated on CD4 + Tregs with the Helios hi gate indicated. (F) Percentage of Helios hi cells among CD4 + Tregs. (G) Representative flow cytometry plots pregated on CD8 + CD127 + Tregs, with the Helios + gate indicated. (H) Percentage of Helios + cells among CD8 + Tregs. The data represent the mean ± SD: *P < 0.05, **P < 0.01; ***,P < 0.001; ****,P < 0.0001 for comparisons between MRL animals. One-way ANOVA with a Tukey multi-comparison post-test was used. Each point represents a single animal: C57BL/6, n = 3 (A, B) , 5 (C, D) , 4 (E, F) , and 5 (G, H) ; prediseased MPJ (pre.MPJ), n = 6 (A, B) , 4 (C, D) , 10 (E, F) , and 4 (G, H) ; diseased MPJ (dis.MPJ), n = 14 (A, B) , 3 (C, D) , 4 (E, F) , and 3 (G, H) ; prediseased LPR (pre.LPR), n = 10 (A, B) , 4 (C, D) , 9 (E, F) , and 4 (G, H) ; and diseased LPR (dis.LPR), n = 18 (A, B) , 6 (C, D) , 12 (E, F) , and 6 (G, H) .

Article Snippet: In the case of flow cytometry for DCs, the cells were harvested in PBS Ethylenediaminetetraacetic acid (EDTA) (5 mM) on day 13, and the following conjugated antibodies were used: B220-efluor450 (RA3-6B2), CCR7-biotin (4B12), CD4-APCef780 (RM4.5), CD8 α -APCef780 (53-6.7), CD11b-Alexaf700 (M1/70), CD11c-APC (N418), CD62L-APC (MEL-14), CD69-PeCy7 (H1.2F3), CD73-PeCy7 (eBioTY/11.8), CD80-PeCy5 (16-10A1), CD122-FITC (fluorescein isothiocyanate) (TM-b1), CD127-PeCy7 (A7R34), FoxP3-PE (FJK-16s), Helios-ef450 (22F6), inducible co-stimulator (ICOS)-FITC (7E.17G9), Ly49-PE (14B11), PDCA-1-PeCy7 (eBio927), RORγ(t)-PE (AFKJS-9), and TCRVα2-PE (B20.1, all from eBioscience); B220-PeCy7 (RA3-6B2), CD3-APC (145-2C11), CD3-PercP (145-2C11), CD4-APC (RM4.5), CD8 α -APC (53-6.7), CD8 α -PercP (53-6.7), CD44-PercP (IM7), CD49b-PE (DX5), CD86-PE (GL-1), CD335(NKp46)-APC (29A1.4), Major Histocompatibility Complex Class II (MHCII)-PE (M5/114.15.2), and PDL-1 (10F.9G2, all from BioLegend); CD11c-FITC (HL3), CD25-biotin (7D4), CD62L-PE (MEL-14), CD244.2-biotin (2B4), TCRβ-PE (H57-597), and TCRγδ-biotin (GL3, all from BD Biosciences); CD3-FITC (145-2C11), CD19-SPRD (6D5), and CD44-FITC (KM201, all from SouthernBiotech); CD244.1-biotin (REA524, Miltenyi Biotec).

Techniques: Expressing, Flow Cytometry, Comparison