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Image Search Results
Journal: Frontiers in Immunology
Article Title: Helios Expression Is Downregulated on CD8 + Treg in Two Mouse Models of Lupus During Disease Progression
doi: 10.3389/fimmu.2022.922958
Figure Lengend Snippet: Helios expression in CD4 + and CD8 + Tregs from MPJ and LPR mice. (A) Representative flow cytometry plots pregated on splenic CD4 + T cells (CD3 + CD8 − CD4 + ). The CD4 + Treg (CD25 + FoxP3 + ) gates are indicated. (B) Percentage of CD4 + Tregs among the CD4 + T cells. (C) Representative flow cytometry plots pregated on splenic CD8 + CD44 + T cells (CD3 + CD8 + CD44 + ), in which the CD8 + Treg (CD122 + Ly49 + ) gates are indicated. (D) Percentage of CD8 + Treg among CD8 + CD44 + T cells. (E) Representative flow cytometry plots pregated on CD4 + Tregs with the Helios hi gate indicated. (F) Percentage of Helios hi cells among CD4 + Tregs. (G) Representative flow cytometry plots pregated on CD8 + CD127 + Tregs, with the Helios + gate indicated. (H) Percentage of Helios + cells among CD8 + Tregs. The data represent the mean ± SD: *P < 0.05, **P < 0.01; ***,P < 0.001; ****,P < 0.0001 for comparisons between MRL animals. One-way ANOVA with a Tukey multi-comparison post-test was used. Each point represents a single animal: C57BL/6, n = 3 (A, B) , 5 (C, D) , 4 (E, F) , and 5 (G, H) ; prediseased MPJ (pre.MPJ), n = 6 (A, B) , 4 (C, D) , 10 (E, F) , and 4 (G, H) ; diseased MPJ (dis.MPJ), n = 14 (A, B) , 3 (C, D) , 4 (E, F) , and 3 (G, H) ; prediseased LPR (pre.LPR), n = 10 (A, B) , 4 (C, D) , 9 (E, F) , and 4 (G, H) ; and diseased LPR (dis.LPR), n = 18 (A, B) , 6 (C, D) , 12 (E, F) , and 6 (G, H) .
Article Snippet: In the case of flow cytometry for DCs, the cells were harvested in PBS Ethylenediaminetetraacetic acid (EDTA) (5 mM) on day 13, and the following conjugated antibodies were used: B220-efluor450 (RA3-6B2), CCR7-biotin (4B12), CD4-APCef780 (RM4.5), CD8 α -APCef780 (53-6.7), CD11b-Alexaf700 (M1/70), CD11c-APC (N418), CD62L-APC (MEL-14), CD69-PeCy7 (H1.2F3), CD73-PeCy7 (eBioTY/11.8), CD80-PeCy5 (16-10A1),
Techniques: Expressing, Flow Cytometry, Comparison